Flow Cytometry Core Facility (FACS)

The Trudeau Institute Flow Cytometry Core Facility, widely recognized as one of the most vital components of our research efforts, currently houses four analyzers and one high-speed cell sorter. 

The analyzers include a four-laser (14 color) BD LSR II, two three-laser (8 color) BD FACSCanto II instruments, and a two-laser (4 color) BD Accuri. Operation of these instruments is available to scientific staff following appropriate training. Analyzer use is available at all hours, seven days a week but must be scheduled in advance.

In addition to maintaining the four investigator-run analyzers, core staff are responsible for cell-sorting duties on a four-laser (11 color) BD Influx cell sorter.  Along with the ability to perform high-speed sorting on up to four distinct populations or sort single cells into tissue culture plates or onto slides, the BD Influx is also equipped with a biosafety cabinet which allows sterile sorting of infectious BSL2 level samples.

Further, the flow cytometry core facility houses a shared use Windows PC powered by an 8-core CPU and Nvidia GeForce GTX 1660 Super GPU for analysis of high content flow cytometry data. Core staff are available for assistance with data analysis and FlowJo licenses are loaned at request.