42 acres in Saranac Lake, New York. 18 campus and off-campus buildings, including dedicated animal breeding facilities and housing for visiting scientists.
Contact: Jon Law, Building Systems Specialist
TRUDEAU INSTITUTE ANIMAL FACILITY
The animal facilities at Trudeau Institute total approximately 22,000 feet of AAALAC accredited animal holding space. This space is divided into three areas; a mouse breeding facility (ABF); experimental animal maintenance facilities (EAM and Stafford Wing); and a segregated ABSL-3 facility.
All animals are housed in individually ventilated micro isolator cages. More than 95% of the mice used each year are bred on site in the mouse breeding facility, which contains more than 200 inbred, congenic, transgenic and mutant strains.
The self contained barrier facility was remodeled in 2002 and has updated HVAC, cagewash, and autoclave equipment, as well as a laboratory for rederivation and cryopreservation of mouse strains. The EAM and Stafford Wing, which are conveniently located adjacent to the research laboratories, have space for 7,800 cages. These facilities house animals for experimentation; mice requiring ABSL-2 and ABSL-3 conditions and a quarantine area. An additional ABSL-3 mouse facility is available for studies involving mouse pathogens.
A comprehensive sentinel monitoring program is in place for all animal facilities.
Contact: Doug Crowell, B.S., Director, Animal Core Facilities
ANIMAL TECHNICAL SERVICES
The Animal Technical Services (ATS) core is responsible for several programs that are essential to providing high quality mice to the Trudeau research community. It oversees the animal health reporting system, which includes examining, reporting and treating animals that are identified with health problems. The core also maintains the re-derivation, cryopreservation, sentinel health monitoring and environmental monitoring programs.
ATS also coordinates the importation and exportation of mice from/to other research facilities or non-commercial vendors. Imported mice go through a rigorous quarantine process and screening which is coordinated by the core. The core coordinates and arranges the testing of biological materials prior to use in the animal facilities.
Staff in the core are extensively trained and experienced with many surgical and laboratory procedures. They offer training to staff in these areas and also oversee the approval and sign-off of new staff in such procedures.
Finally, Animal Technical Services provides technical support for research projects on a fee-for-service basis. These services are intended to supplement those provided by technical staff in the individual research laboratories where the expertise does not exist or where a temporary augmentation of research staff is required.
Contact: Jody Hart, B.S., Technical Services Manager
MOUSE PRODUCTION SCREENING SERVICES (MPSS)
The Mouse Production Screening Service (MPSS) manages mouse breeding at the Trudeau Institute. Trudeau scientists use experimental mice in large numbers, and mice are an essential institute resource. MPSS staff members closely monitor investigator requirements for mice, and adjust numbers of breeders to meet those demands. The MPSS maintains extensive computerized records of mice issued to investigators and of mouse inventories, including that of aged mice, which comprise a substantial fraction of the institute’s total mouse population.
The MPSS oversees nearly 200 strains of mice in the institute’s stand-alone Animal Breeding Facility and Experimental Animal Module in the main laboratory. Strains include inbreds, hybrids, and many transgenic and gene-targeted mutant mice. MPSS staff members also perform genetic screening using flow cytometric and PCR-based assays. MPSS closely coordinates its activities with those of the Animal Facility, which has responsibility for caretaking and animal husbandry, and Animal Technical Services, which oversees the health and welfare of the mice.
Contact: Doug Crowell, B.S., Director, Animal Core Facilities
BIOSAFETY PROGRAM AND CONTAINMENT FACILITIES
The Biosafety program, as a component of the Trudeau Institute’s overall Occupational Health and Safety Program, exists to protect the safety and well being of employees at risk for occupational exposure to infectious agents. Engineering and exposure controls as well as specialized work practices are in place to prevent occupational acquired illness.
The Biosafety Officer (BSO) and the Institutional Biosafety Committee (IBC) oversee and provide advice on research conducted with infectious agents.
The IBC follows the policies and procedures set forth in the National Institutes of Health (NIH) Guidelines for Research Involving Recombinant DNA Molecules and in Biosafety in Microbiological and Biomedical Laboratories 5th Ed.
Our biosafety level 2 (BSL2) and biosafety level 3 (BSL3) laboratories and animal facilities are designed to meet the guidelines for work with various infectious agents. To date, our BSL3 facilities have been used exclusively for research on Mycobacterium tuberculosis, however, after certification from the NIH and the Centers for Disease Control and Prevention (CDC) our newest state-of-the-art BSL3 containment facility will be available for research on both Mycobacterium tuberculosis and Yersinia pestis.
Contact: Jennifer Kazmierczak, M.S., Occupational Health and Safety Manager
FLOW CYTOMETRY CORE FACILITY (FACS)
The Trudeau Institute FACS facility, widely recognized as one of the most vital components of our research efforts, currently houses six analyzers and one high-speed cell sorter. The analyzers include one four laser 14 color BD LSR II, one three-laser, eight color BD FACSCanto II, two dual-laser four color BD FACSCaliburs, one three-color BD FACScan, and one modified two laser, five-color BD FACScan.
Operation of these instruments is available to scientific staff following appropriate training. Analyzer use is available at all hours, seven days a week but must be scheduled in advance. In addition to maintaining the six investigator-run analyzers, the Flow Cytometry Core Facility staff is also responsible for cell-sorting duties on a three-laser eight color BD FACS Vantage w/DiVa option. Along with the ability to perform high speed sorting on up to four distinct populations or sort single cells into tissue culture plates and onto slides, the FACS Vantage is also equipped with a Class I biosafety hood which allows sorting of BSL2 level infectious samples.
Cell sorting is normally performed during standard business hours and must be scheduled in advance with the FACS Facility manager.
Contact: Ronald LaCourse, A.A.S., FACS Facility Manager
HISTOLOGY / IMAGING CORE
The Histology/Imaging core provides support to the institute staff in the areas of Histology, Immunohistochemistry, Immunofluorescence, and Imaging. Specifically, staff members of the core provide management of imaging resources, training in the use of core equipment, and direct technical support. The technical support can include full performance of protocols as well as development of new techniques and protocols as required.
Contact: Mike Tighe, A.A.S., Imaging and Histology Core Manager
The IT core provides shared computer support through an Institute-wide computer network via wireless and hard-wired computers. The network supports an Institute-wide intranet that allows users to access institute administrative and core services (animal, library, FACS, molecular biology and imaging cores). The IT core also provides central file servers for data management as well as data backup and archiving. The IT core also provides centralized software licensing, remote communication services, local support for seminars and meetings. The core also maintains a computer helpdesk and repair service.
Contact: Kenneth J. Whitelaw, B.S., Director of Information and Research Technology
MOLECULAR BIOLOGY CORE FACILITY (MBCF)
The molecular biology core facility supports research at the Trudeau Institute by providing molecular biology-oriented services, products, shared equipment and consultation in such areas as automated DNA sequencing, Real-time PCR, spectratyping, RNA and protein analysis using microfluidics and production of recombinant proteins. In addition, the core generates MHC class I and class II tetrameric reagents for the identification of antigen specific CD8+ and CD4+ T cells.
Contact: Gail Huston, M.S., Molecular Biology Core Facility, Technical Specialist
MONOCLONAL ANTIBODY CORE
The Monoclonal Antibody core facility at Trudeau Institute provides staff with low-cost, high-quality antibody reagents. We primarily provide antibodies for in vivo studies, which require high-quality reagents in relatively large quantities.
Thus, we procure commonly used antibodies in bulk and then re-sell them at cost in smaller aliquots. We also reduce expenditures by performing quality control tests on the bulk material (i.e. assays for endotoxin and other biological contaminants). Our current inventory includes antibodies targeting the mouse gamma-interferon, tumor necrosis factor, interleukin-4, interleukin-6, interleukin-12, interleukin-6 receptor, CD4, CD8, CD25, CD16/32, CD90, NK1.1, Ly6G, along with a variety of isotype-matched control antibodies.
Contact: Steve Smiley, Ph.D
TRUDEAU INSTITUTE DATA ANALYSIS RESOURCE (TIDAR)
TIDAR is an informal resource to provide Trudeau scientists with advice and information about experimental design and statistical data analysis. The resource helps Trudeau staff with handling of quantitative data including power calculations, choice of appropriate statistical tests, use of data transformations, and design of experiments. The resource is available at no cost to all staff as they assess experimental results and prepare grant proposals and manuscripts.
Contact: Larry Johnson, Ph.D.
TRUDEAU ADMINISTRATIVE SERVICES
The Institute provides a full-range of administrative services in support of scientific research. We have a well-trained staff that provides services in the following areas:
· Pre-award and post-award grant administration
· Human resources, including visa preparation, salary and benefits
administration, employee relations and counseling
· Travel services for meetings and conferences
· Housing for postdoctoral fellows and visiting scientists
· Library and information services
· Occupational health and safety, including support for radiation services,
biosafety and laboratory operations
· Building and instrumentation maintenance and security
· Purchasing, shipping and receiving
· Accounting and payroll services
· Reception and secretarial services
Contact: Suzanne Chapman, A.A.S., Assistant to the COO